{"id":3451,"date":"2025-03-10T11:12:40","date_gmt":"2025-03-10T11:12:40","guid":{"rendered":"https:\/\/www.sus-mirri.it\/2025\/03\/inhibitory-effects-of-nisin-and-gallium-iii-nitrate-hydrate-on-planktonic-and-adhered-cells-and-implications-for-the-viable-but-non-culturable-state\/"},"modified":"2025-03-10T11:13:53","modified_gmt":"2025-03-10T11:13:53","slug":"inhibitory-effects-of-nisin-and-gallium-iii-nitrate-hydrate-on-planktonic-and-adhered-cells-and-implications-for-the-viable-but-non-culturable-state","status":"publish","type":"post","link":"https:\/\/www.sus-mirri.it\/it\/2025\/03\/inhibitory-effects-of-nisin-and-gallium-iii-nitrate-hydrate-on-planktonic-and-adhered-cells-and-implications-for-the-viable-but-non-culturable-state\/","title":{"rendered":"Inhibitory Effects of Nisin and Gallium (III) Nitrate Hydrate on Planktonic and Adhered Cells and Implications for the Viable but Non-Culturable State"},"content":{"rendered":"\n<p>Poscente, V.; Di Gregorio, L.; Bernini, R.; Bevivino, A. 2025 Inhibitory Effects of Nisin and Gallium (III) Nitrate Hydrate on Planktonic and Adhered Cells and Implications for the Viable but Non-Culturable State Microorganisms 13(2): 276\u00a0<a href=\"https:\/\/doi.org\/10.3390\/microorganisms13020276\">https:\/\/doi.org\/10.3390\/microorganisms13020276<\/a><\/p>\n\n\n\n<p>ABSTRACT<\/p>\n\n\n\n<p>Effective antimicrobial and biofilm control strategies require an understanding of the differential effects of antimicrobial agents on the viability and culturability of microbial cells. A viable but non-culturable (VBNC) state, a survival strategy of non-spore-forming bacteria in response to adverse conditions, poses a significant challenge for public health and food safety. In the present study, we investigated the antimicrobial and antibiofilm effects of nisin and gallium (III) nitrate hydrate against the Gram-positive strain\u00a0<em>Lactiplantibacillus plantarum<\/em>\u00a0subsp.\u00a0<em>plantarum<\/em>\u00a0DSM 20174 and the Gram-negative strain\u00a0<em>Pseudomonas fluorescens<\/em>\u00a0ATCC 13525, respectively. Both strains were chosen as model systems for their relevance to food and clinical settings. Culture-based methods and flow cytometry (FCM) were used to evaluate the culturability and viability of both planktonic and sessile cells, providing insights into their physiological response to antimicrobial treatment-induced stress at different concentrations (100, 250, 350, and 500 ppm). The findings highlight the strain-specific action of nisin on\u00a0<em>L. plantarum<\/em>\u00a0and the promising antibiofilm effects of Ga (III) against\u00a0<em>P. fluorescens<\/em>. This study underscores the promising potential of FCM as a powerful tool for high-throughput analyses of antimicrobial efficacy, providing valuable insights into developing targeted biofilm control strategies for food safety and clinical applications.<\/p>\n\n\n\n<p>Read more at: <a href=\"https:\/\/www.mdpi.com\/2076-2607\/13\/2\/276\">https:\/\/www.mdpi.com\/2076-2607\/13\/2\/276<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Poscente, V.; Di Gregorio, L.; Bernini, R.; Bevivino, A. 2025 Inhibitory Effects of Nisin and Gallium (III) Nitrate Hydrate on Planktonic and Adhered Cells and Implications for the Viable but Non-Culturable State Microorganisms 13(2): 276\u00a0https:\/\/doi.org\/10.3390\/microorganisms13020276 ABSTRACT Effective antimicrobial and biofilm control strategies require an understanding of the differential effects of antimicrobial agents on the viability [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":3449,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_uag_custom_page_level_css":"","footnotes":""},"categories":[58],"tags":[71,108,64],"class_list":["post-3451","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news-it","tag-mirri-it","tag-pubblications-it","tag-sus-mirri-it"],"uagb_featured_image_src":{"full":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI.png",1080,1080,false],"thumbnail":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-150x150.png",150,150,true],"medium":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-300x300.png",300,300,true],"medium_large":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-768x768.png",768,768,true],"large":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-1024x1024.png",1024,1024,true],"1536x1536":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI.png",1080,1080,false],"2048x2048":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI.png",1080,1080,false],"blade-grve-large-rect-horizontal":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-1080x658.png",1080,658,true],"blade-grve-small-square":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-600x600.png",600,600,true],"blade-grve-small-rect-horizontal":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-800x600.png",800,600,true],"blade-grve-small-rect-horizontal-wide":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-800x450.png",800,450,true],"blade-grve-small-rect-vertical":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-600x800.png",600,800,true],"blade-grve-medium-rect-vertical":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-560x1080.png",560,1080,true],"blade-grve-medium-square":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI.png",1080,1080,false],"blade-grve-fullscreen":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI.png",1080,1080,false]},"uagb_author_info":{"display_name":"Maria Gullo","author_link":"https:\/\/www.sus-mirri.it\/it\/author\/maria-gullo\/"},"uagb_comment_info":0,"uagb_excerpt":"Poscente, V.; Di Gregorio, L.; Bernini, R.; Bevivino, A. 2025 Inhibitory Effects of Nisin and Gallium (III) Nitrate Hydrate on Planktonic and Adhered Cells and Implications for the Viable but Non-Culturable State Microorganisms 13(2): 276\u00a0https:\/\/doi.org\/10.3390\/microorganisms13020276 ABSTRACT Effective antimicrobial and biofilm control strategies require an understanding of the differential effects of antimicrobial agents on the viability&hellip;","_links":{"self":[{"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/posts\/3451","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/comments?post=3451"}],"version-history":[{"count":1,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/posts\/3451\/revisions"}],"predecessor-version":[{"id":3452,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/posts\/3451\/revisions\/3452"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/media\/3449"}],"wp:attachment":[{"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/media?parent=3451"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/categories?post=3451"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/tags?post=3451"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}