{"id":3475,"date":"2025-03-17T10:36:55","date_gmt":"2025-03-17T10:36:55","guid":{"rendered":"https:\/\/www.sus-mirri.it\/2025\/03\/flow-cytometry-unravelling-the-real-antimicrobial-and-antibiofilm-efficacy-of-natural-bioactive-compounds\/"},"modified":"2025-03-17T10:37:12","modified_gmt":"2025-03-17T10:37:12","slug":"flow-cytometry-unravelling-the-real-antimicrobial-and-antibiofilm-efficacy-of-natural-bioactive-compounds","status":"publish","type":"post","link":"https:\/\/www.sus-mirri.it\/it\/2025\/03\/flow-cytometry-unravelling-the-real-antimicrobial-and-antibiofilm-efficacy-of-natural-bioactive-compounds\/","title":{"rendered":"Flow cytometry: Unravelling the real antimicrobial and antibiofilm efficacy of natural bioactive compounds"},"content":{"rendered":"\n

Poscente, V.; Di Gregorio, L.; Costanzo, M.; Bernini, R.; Bevivino, A. 2024 Flow cytometry: Unravelling the real antimicrobial and antibiofilm efficacy of natural bioactive compounds Journal of Microbiological Methods 222: 106956\u00a0https:\/\/doi.org\/10.1016\/j.mimet.2024.106956<\/a><\/p>\n\n\n\n

Abstract<\/h2>\n\n\n\n

Flow cytometry (FCM) provides unique information on bacterial viability and physiology, allowing a real-time early warning antimicrobial and antibiofilm monitoring system for preventing the spread risk of foodborne disease<\/a>. The present work used a combined culture-based and FCM approach to assess the in vitro efficacy of essential oils (EOs) from condiment<\/a> plants commonly used in Mediterranean Europe (i.e., thyme EO, oregano<\/a> EO, basil<\/a> EO, and lemon EO) against planktonic and sessile cells of food-pathogenic Listeria monocytogenes<\/a> 56 LY, and contaminant and alterative species Escherichia coli<\/a> ATCC 25922 and Pseudomonas fluorescens<\/a> ATCC 13525. Evaluation of the bacterial response to the increasing concentrations of natural compounds posed FCM as a crucial technique for the quantification of the live\/dead, and viable but non-culturable (VBNC) cells when antimicrobial agents exert no real bactericidal action. Furthermore, the FCM results displayed higher numbers of viable bacteria expressed as Active Fluorescent Units (AFUs) with a greater level of repeatability compared with outcomes of the plate-count method. Overall, accurate counting of viable microbial cells is a critically important parameter in food microbiology<\/a>, and flow cytometry provides an innovative approach with high-throughput potential for applications in the food industry as \u201cflow microbiology\u201d.<\/p>\n\n\n\n

Read more at: https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S016770122400068X?via%3Dihub<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Poscente, V.; Di Gregorio, L.; Costanzo, M.; Bernini, R.; Bevivino, A. 2024 Flow cytometry: Unravelling the real antimicrobial and antibiofilm efficacy of natural bioactive compounds Journal of Microbiological Methods 222: 106956\u00a0https:\/\/doi.org\/10.1016\/j.mimet.2024.106956 Abstract Flow cytometry (FCM) provides unique information on bacterial viability and physiology, allowing a real-time early warning antimicrobial and antibiofilm monitoring system for preventing […]<\/p>\n","protected":false},"author":5,"featured_media":3473,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_uag_custom_page_level_css":"","footnotes":""},"categories":[58],"tags":[71,108,64],"class_list":["post-3475","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news-it","tag-mirri-it","tag-pubblications-it","tag-sus-mirri-it"],"uagb_featured_image_src":{"full":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-2.png",1080,1080,false],"thumbnail":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-2-150x150.png",150,150,true],"medium":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-2-300x300.png",300,300,true],"medium_large":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-2-768x768.png",768,768,true],"large":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-2-1024x1024.png",1024,1024,true],"1536x1536":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-2.png",1080,1080,false],"2048x2048":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-2.png",1080,1080,false],"blade-grve-large-rect-horizontal":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-2-1080x658.png",1080,658,true],"blade-grve-small-square":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-2-600x600.png",600,600,true],"blade-grve-small-rect-horizontal":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-2-800x600.png",800,600,true],"blade-grve-small-rect-horizontal-wide":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-2-800x450.png",800,450,true],"blade-grve-small-rect-vertical":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-2-600x800.png",600,800,true],"blade-grve-medium-rect-vertical":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-2-560x1080.png",560,1080,true],"blade-grve-medium-square":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-2.png",1080,1080,false],"blade-grve-fullscreen":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2025\/03\/SUS-MIRRI-2.png",1080,1080,false]},"uagb_author_info":{"display_name":"Maria Gullo","author_link":"https:\/\/www.sus-mirri.it\/it\/author\/maria-gullo\/"},"uagb_comment_info":0,"uagb_excerpt":"Poscente, V.; Di Gregorio, L.; Costanzo, M.; Bernini, R.; Bevivino, A. 2024 Flow cytometry: Unravelling the real antimicrobial and antibiofilm efficacy of natural bioactive compounds Journal of Microbiological Methods 222: 106956\u00a0https:\/\/doi.org\/10.1016\/j.mimet.2024.106956 Abstract Flow cytometry (FCM) provides unique information on bacterial viability and physiology, allowing a real-time early warning antimicrobial and antibiofilm monitoring system for preventing…","_links":{"self":[{"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/posts\/3475","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/comments?post=3475"}],"version-history":[{"count":1,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/posts\/3475\/revisions"}],"predecessor-version":[{"id":3476,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/posts\/3475\/revisions\/3476"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/media\/3473"}],"wp:attachment":[{"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/media?parent=3475"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/categories?post=3475"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/tags?post=3475"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}