{"id":3991,"date":"2026-02-12T09:15:18","date_gmt":"2026-02-12T09:15:18","guid":{"rendered":"https:\/\/www.sus-mirri.it\/2026\/02\/screening-purification-and-characterization-of-protease-inhibitors-from-different-legume-seeds-with-cytotoxic-and-antimicrobial-effects\/"},"modified":"2026-02-12T09:19:03","modified_gmt":"2026-02-12T09:19:03","slug":"screening-purification-and-characterization-of-protease-inhibitors-from-different-legume-seeds-with-cytotoxic-and-antimicrobial-effects","status":"publish","type":"post","link":"https:\/\/www.sus-mirri.it\/it\/2026\/02\/screening-purification-and-characterization-of-protease-inhibitors-from-different-legume-seeds-with-cytotoxic-and-antimicrobial-effects\/","title":{"rendered":"Screening, Purification, and Characterization of Protease Inhibitors From Different Legume Seeds With Cytotoxic and Antimicrobial Effects"},"content":{"rendered":"\n<p>Ibrahim, M. M., Cavallero, A., Zaki, E. R., Rady, M. R., Sbrana, C., &amp; Gabriele, M. 2025 Screening, Purification, and Characterization of Protease Inhibitors From Different Legume Seeds With Cytotoxic and Antimicrobial Effects. Legume Science 7(4), e70058\u00a0<a href=\"https:\/\/doi.org\/10.1002\/leg3.70058\">https:\/\/doi.org\/10.1002\/leg3.70058<\/a><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"d142206614\">ABSTRACT<\/h2>\n\n\n\n<p>This study reports the purification and characterization of protease inhibitors (PIs) from three Egyptian legumes: soybean (<em>Glycine max<\/em>), cowpea (<em>Vigna unguiculata<\/em>), and chickpea (<em>Cicer arietinum<\/em>). Their antimicrobial and cytotoxic activities were also assessed. PIs were extracted using different buffers: 0.02\u2009M HCl yielded the highest activity for soybean and cowpea, while 0.3\u2009M NaCl was optimal for chickpea. Ammonium sulfate precipitation at 0%\u201390% saturation was best for soybean and chickpea, whereas 0%\u201360% was most effective for cowpea. Purification via DEAE-Sepharose showed that 0.25\u2009M NaCl fractions had the highest total and specific activity across all legumes. Sephadex G-50 further enhanced specific PIs activity. Electrophoresis revealed bands at 25\u2009kDa (soybean), 45\u2009kDa (cowpea), and 35\u2009kDa (chickpea). PIs showed variable cytotoxicity depending on legume, lyophilization, cell line, and exposure time. Lyophilized soybean PIs significantly reduced human hepatocellular carcinoma (HepG2) cell viability after 48\u2009h, while nonlyophilized chickpea PIs also affected HepG2. Cowpea PIs notably induced human colorectal adenocarcinoma (HT-29) cell death at higher concentrations; instead, human lung adenocarcinoma (A549) cells were generally less sensitive, except at high cowpea PI doses. All lyophilized PIs inhibited&nbsp;<em>Bacillus subtilis<\/em>&nbsp;but were inactive against&nbsp;<em>Staphylococcus epidermidis<\/em>&nbsp;and&nbsp;<em>Pseudomonas stutzeri<\/em>.<\/p>\n\n\n\n<p>Read more at: <a href=\"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/leg3.70058\">https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/leg3.70058<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Ibrahim, M. M., Cavallero, A., Zaki, E. R., Rady, M. R., Sbrana, C., &amp; Gabriele, M. 2025 Screening, Purification, and Characterization of Protease Inhibitors From Different Legume Seeds With Cytotoxic and Antimicrobial Effects. Legume Science 7(4), e70058\u00a0https:\/\/doi.org\/10.1002\/leg3.70058 ABSTRACT This study reports the purification and characterization of protease inhibitors (PIs) from three Egyptian legumes: soybean (Glycine [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":3989,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_uag_custom_page_level_css":"","footnotes":""},"categories":[58],"tags":[71,108,64],"class_list":["post-3991","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news-it","tag-mirri-it","tag-pubblications-it","tag-sus-mirri-it"],"uagb_featured_image_src":{"full":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2026\/02\/SUS-MIRRI-2.png",1080,1080,false],"thumbnail":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2026\/02\/SUS-MIRRI-2-150x150.png",150,150,true],"medium":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2026\/02\/SUS-MIRRI-2-300x300.png",300,300,true],"medium_large":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2026\/02\/SUS-MIRRI-2-768x768.png",768,768,true],"large":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2026\/02\/SUS-MIRRI-2-1024x1024.png",1024,1024,true],"1536x1536":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2026\/02\/SUS-MIRRI-2.png",1080,1080,false],"2048x2048":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2026\/02\/SUS-MIRRI-2.png",1080,1080,false],"blade-grve-large-rect-horizontal":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2026\/02\/SUS-MIRRI-2-1080x658.png",1080,658,true],"blade-grve-small-square":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2026\/02\/SUS-MIRRI-2-600x600.png",600,600,true],"blade-grve-small-rect-horizontal":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2026\/02\/SUS-MIRRI-2-800x600.png",800,600,true],"blade-grve-small-rect-horizontal-wide":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2026\/02\/SUS-MIRRI-2-800x450.png",800,450,true],"blade-grve-small-rect-vertical":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2026\/02\/SUS-MIRRI-2-600x800.png",600,800,true],"blade-grve-medium-rect-vertical":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2026\/02\/SUS-MIRRI-2-560x1080.png",560,1080,true],"blade-grve-medium-square":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2026\/02\/SUS-MIRRI-2.png",1080,1080,false],"blade-grve-fullscreen":["https:\/\/www.sus-mirri.it\/wp-content\/uploads\/2026\/02\/SUS-MIRRI-2.png",1080,1080,false]},"uagb_author_info":{"display_name":"Maria Gullo","author_link":"https:\/\/www.sus-mirri.it\/it\/author\/maria-gullo\/"},"uagb_comment_info":0,"uagb_excerpt":"Ibrahim, M. M., Cavallero, A., Zaki, E. R., Rady, M. R., Sbrana, C., &amp; Gabriele, M. 2025 Screening, Purification, and Characterization of Protease Inhibitors From Different Legume Seeds With Cytotoxic and Antimicrobial Effects. Legume Science 7(4), e70058\u00a0https:\/\/doi.org\/10.1002\/leg3.70058 ABSTRACT This study reports the purification and characterization of protease inhibitors (PIs) from three Egyptian legumes: soybean (Glycine&hellip;","_links":{"self":[{"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/posts\/3991","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/comments?post=3991"}],"version-history":[{"count":1,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/posts\/3991\/revisions"}],"predecessor-version":[{"id":3992,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/posts\/3991\/revisions\/3992"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/media\/3989"}],"wp:attachment":[{"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/media?parent=3991"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/categories?post=3991"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.sus-mirri.it\/it\/wp-json\/wp\/v2\/tags?post=3991"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}