Contaldo SG, d’Acierno A, Bosio L, Venice F, Perottino EL, Hoyos Rea JE, Varese GC, Cordero F, Beccuti M 2025 Long-read microbial genome assembly, gene prediction and functional annotation: a service of the MIRRI ERIC Italian node Frontiers Bioinformatics 10.3389/fbinf.2025.1632189
Background: Understanding the structure and function of microbial genomes is crucial for uncovering their ecological roles, evolutionary trajectories, and potential applications in health, biotechnology, agriculture, food production, and environmental science. However, genome reconstruction and annotation remain computationally demanding and technically complex.
Results: We introduce a bioinformatics platform designed explicitly for long-read microbial sequencing data to address these challenges. Developed as a service of the Italian MIRRI ERIC node, the platform provides a comprehensive solution for analyzing both prokaryotic and eukaryotic genomes, from assembly to functional protein annotation. It integrates state-of-the-art tools (e.g., Canu, Flye, BRAKER3, Prokka, InterProScan) within a reproducible, scalable workflow built on the Common Workflow Language and accelerated through high-performance computing infrastructure. A user-friendly web interface ensures accessibility, even for non-specialists.
Read more at: https://www.frontiersin.org/journals/bioinformatics/articles/10.3389/fbinf.2025.1632189/full